Coding

Part:BBa_K2130014

Designed by: Ooi Kean Hean   Group: iGEM16_NTU-Singapore   (2016-10-24)


SaCas9

This past is a human condon optimised Staph. aureus Cas9. SaCas9 recognises an 5'-NGGRRT-3' PAM at the 3' end of the target sequence. Example(PAM in brackets):

5'-NNNN...NNN(NGGRRT)-3' Evaluation studies from our team have compared the editing efficiency of this part compared to other Cas9/Cpf1.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1193
    Illegal BglII site found at 1680
    Illegal BglII site found at 2303
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 139
    Illegal NgoMIV site found at 3213
    Illegal NgoMIV site found at 3232
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None